Background pattern of a brain with neural connections
Su-Chun Zhang

Su-Chun Zhang

Co-PI (Core Leadership)

University of Wisconsin-Madison

Su-Chun Zhang received his MD (1984) and MS (1989) in China, and PhD (1996) in Canada. Following postdoctoral training, he began his assistant professor in 2001 and is now Steenbock Professor in Behavioral and Neural Sciences at University of Wisconsin-Madison. He is also professor and director of the Signature Program in Neuroscience and Behavioral Disorders at Duke-NUS Medical School, Singapore. His group has developed technology to guide human pluripotent stem cells to functionally specialized nerve cell types. He is dissecting mechanisms of and developing therapeutics for neurological conditions like Parkinson’s disease. Dr. Zhang is a founder of the WiCell Institute and BrainXell, Inc.

Teams

Scherzer

Themes

PD Functional Genomics

Tags

GWAS PD progression RNA-seq

Recent ASAP Preprints & Published Papers

FM1-43 endocytic uptake assay in HIPSC derived neurons

FM1-43 is a lipophilic styryl dye, nontoxic to the cells, virtually nonfluorescent in aqueous media. The dye will become fluorescent once it binds to the plasma membrane. In neurons that are actively releasing neurotransmitters, these dyes become internalized within the recycled synaptic vesicles, and the nerve terminals become brightly stained. This shows an increase in the fluorescence intensity of FM1-43. When the cells undergo another stimulation (through KCL or electrical) the FM1-43 dye containing vesicles will be released through exocytosis. Thus, resulting in a decrease in the FM1-43 fluorescence intensity.

Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons

This protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.

Team Scherzer

Clemens Scherzer

Clemens Scherzer

Lead PI (Core Leadership)

Su-Chun Zhang

Su-Chun Zhang

Co-PI (Core Leadership)

Joshua Levin

Joshua Levin

Co-PI (Core Leadership)

Mel Feany

Mel Feany

Co-PI (Core Leadership)

Xianjun Dong

Xianjun Dong

Co-PI (Core Leadership)

Beatrice Weykopf

Beatrice Weykopf

Project Manager

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