Raja Sekhar Nirujogi
Co-PI (Core Leadership)
Key Personnel
University of Dundee
Raja is an Independent Investigator at Medical Research Council Protein Phosphorylation and Ubiquitylation Unit at University of Dundee. He contributed to the identification of LRRK2 kinase substrates and developed targeted mass spectrometry assays and organelle proteomic methodologies. His current research is focused on applying single cell proteomic methodologies to better understand PD brain disease heterogeneity.
Teams
Themes
Tags
Ciliogenesis
LRRK2
Lysosomes
PINK1
Rab
GBA (Glucocerebrosidase)
Rab GTPases
Cell biology
Neuroprotective/regenerative
Lysosomal dysfunction
Endolysosomal dysfunction
LRRK2 pathway
PD heterogeneity: clearance
Recent ASAP Preprints & Published Papers
Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples
Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling lysosomal content using tag-based lysosomal immunoprecipitation (LysoTagIP) in cell and animal models allowed major discoveries in the field, however studying lysosomal dysfunction in human patients remains challenging. Here, we…
CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data
To facilitate analysis and sharing of mass spectrometry (MS)-based proteomics data, we created online tools called CURTAIN (https://curtain.proteo.info) and CURTAIN-PTM (https://curtainptm.proteo.info) with an accompanying series of video tutorials (https://www.youtube.com/@CURTAIN-me6hl). These are designed to enable non-MS experts to interactively peruse volcano plots and deconvolute primary experimental data so that replicates can…
