Johan Jakobsson
Lead PI (Core Leadership)
Co-PI (Core Leadership)
Lund University
Johan Jakobsson is a Professor in the Department of Experimental Medical Sciences at Lund University, Sweden and the Director of the Lund Stem Cell Center. He studied gene therapy in the brain as a graduate student in Lund and performed his postdoctoral work with Didier Trono at EPFL focusing on transposable elements.
Teams
Themes
Tags
iPSCs (Induced pluripotent stem cells)
Transposable elements
CRISPR
Neuroinflammation
Aging
Epigenomics
Disease heterogeneity
PD heterogeneity: aging
Recent ASAP Preprints & Published Papers
TEsingle enables locus-specific transposable element expression analysis at single-cell resolution
Accurately assessing transposable elements (TE) expression from individual genomic TE locus remains an open problem in the field, due to the highly repetitive nature of these multi-copy sequences. These issues are compounded in single-cell and single-nucleus transcriptome experiments, where additional complications arise due to sparse read coverage and unprocessed mRNA…
Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
The published protocol describes the workflow for transcriptional silencing of transposable elements (TEs) in human induced pluripotent stem cells (hiPSCs). The protocol illustrates how to design gRNAs to target families of TEs (or a unique TE locus) and how to validate the efficiency and specificity of large-scale CRISPRi-based silencing using…
